ACTIVITY OF ETANOL EXTRACT OF SNAKE LID LEAVES (Hedyotis corymbosa (L.) Lamk) ON THE GROWTH OF Staphylococcus aureus BACTERIES
Abstract
Hedyotis corymbosa or commonly known as pearl grass is one of the plants that has great potential as a traditional medicine, especially in the treatment of infections. The development of infection drugs is very rapid considering that the resistance rate in several countries continues to increase. Some cases of infection that often occur are skin infections caused by Staphylococcus aureus bacteria. This study aims to test the ability of ethanol extract of snake tongue leaves in inhibiting the growth of Staphylococcus aureus bacteria. This study is an experimental study, consisting of five treatments with three repetitions, using the paper disk method. Samples in the form of snake tongue leaf extract (Hedyotis corymbosa (L.) Lamk) were dissolved with aqua pro injection which was divided into several concentration groups, namely 5%, 10%, and 15%. The test results showed that snake tongue leaf extract was able to inhibit the growth of Staphylococcus aureus bacteria with an average inhibition area of 13.8 mm (5%), 16.7 mm (10%) and 17.4 mm (15%). Statistical analysis through ANOVA testing obtained results that the three concentrations of snake tongue leaf extract (Hedyotis corymbosa (L.) Lamk) have significant differences in inhibiting the growth of Staphylococcus aureus bacteria.
References
Agustrina, G. 2011. Potential of Propolis of Apis Mellifera Spp as Antibacterial Material. Department of Biochemistry. Faculty of Mathematics and Natural Sciences. Bogor Agricultural University.
Ballitro, N.S. 2014, Potential of Pearl Grass (Hedyotis corymbosa) as anticancer, Warta of industrial plant research and development, volume 20 number 3.
Brooks, G.F., Butel, J.S., Morse, S.A. 2005, Medical Microbiology, Translator: Mudihardi, E., Kuntaman, Wasito, E.B., Salemba Medika, Jakarta.
Dalimartha, S. 2008, Atlas of Indonesian medicinal plants: revealing the richness of Indonesian medicinal plants, Volume 5, Niaga Swadaya, Indonesia.
Indonesian Ministry of Health. 1989. Medical Bacteriology. Jakarta: Ministry of Health of the Republic of Indonesia.
Indonesian Ministry of Health. 1995. Indonesian Pharmacopoeia IV Edition. Ministry of Health of the Republic of Indonesia: Jakarta.
Lestari, Endang S, 2009. Antimicrobial Resistance in Indonesia: Prevalence, determinants and genetic basis. Erasmus MC. University Medical Center Rotterdam.
Hapsari, Endah. 2015. Antibacterial Test of Herba Meniran Extract (Phyllanthus niruri) on the Growth of Bacillus cereus and Escherichia coli Bacteria. Thesis. Biology Education. Sanata Dharma University. Yogyakarta.
Hariana, A. 2006. Medicinal Plants and Their Efficacy, Series 3. Penebar Swadaya. Jakarta.
Ipteknet. 2005. Pearl Grass. Accessed February 16, 2017, http://www.iptek.net.id.ind/.
Jawetz, E., G.E. Melnick, C.A. Adelberg. 2005. Medical Microbiology. Book 2. Translated by dr. Nani Widorini.Publisher of Salemba Medika. Jakarta.
Milah, L.M., Udin, Z., and Wati, E.L .2012, "Test of Antibacterial Activity of Pearl Grass Extract (Hedyotis corymbosa (L.) Lamk".Chemistry study program, Muhammadiyah University, Sukabumi.
Muhlisah, F. 2005. Indonesian National Medicinal Plants, Food and Drug Administration of the Republic of Indonesia, Jakarta.
Nurhayati, Latief, M., and Handoko, H. 2006, "Antimicrobial Test of Pearl Grass (Hedyotis corymbosa) against Bacteria and Fungi Causing Disease in Poultry", Faculty of Agriculture, Jambi University, Jambi.
Pelczar MJJr and Chan ECS. 1998. Fundamentals of Microbiology 2. Jakarta: University of Indonesia Press. 443 pp.
Pendleton, J. 2009. Ursolic Acid: Excitement in Allergy, Inflammation, and Cancer Management. Accessed February 16, 2017, http:herbalproperties.suite101.com/article.cfm/ursolic_acid.
Razak, A., Aziz Djamal, and Gusti Revilla. 2013. Inhibition Test of Lime Fruit Juice (Citrus aurantifolia s.) Against the Growth of Staphylococcus Aureus Bacteria In Vitro, Faculty of Medicine, Andalas University, Padang.
Saptarini, O. 2011. Antibacterial Activity Test of Ethanolic Extracts of Patikan Kebo (Euphorbia hirta Linn) and Herba Rumput Mutiara (Hedyotis corymbosa L) against Pneumonia-Causing Bacteria.
Saraswati. Faradhila. Nur. 2015. Antibacterial Activity Test of 96% Ethanol Extract of Yellow Kepok Banana Peel Waste (Musa balbisiana) Against Acne-Causing Bacteria (Staphylococcus epidermidis, Staphylococcus aureus, and Propionibacterium acne). Jakarta. Faculty of Medicine and Health Sciences.
Seniwati. 2014. Phytochemical screening of alang-alang (imperata cylindrica l.beauv) and snake tongue (Hedyotis corymbosa l.lamk), Lambung Mangkurat University, Banjarbaru.
Siregar, A. F., Sabdono, A., & Pringgenies, D. 2012. Antibacterial Potential of Seaweed Extract against Skin Disease Bacteria Pseudomonas aeruginosa, Staphylococcus epidermidis, and Micrococcus luteus.Journal of marine research, 1(2), 152-160.
Sudirman FY. 2014. Effectiveness Test of Salam Leaf Extract against the growth of Staphylococcus aureus bacteria in vitro. Makassar.UNHAS.
Volk, W.A. Wheeler. 1988. Basic Microbiology. Erlangga: Jakarta.
Wijayakusuma, H. 2008, Indonesian Medicinal Plants, Pustaka Bundai, Jakarta.
